rab4 rfp Search Results


rfp rab4  (Addgene inc)
92
Addgene inc rfp rab4
WIPI1[Sloop] is preferentially associated with recycling endosomes. (A) Colocalization with RAB proteins. Live cell images of HK2 cells expressing EGFP-WIPI1[Sloop], mCherry-RAB5, <t>RFP-RAB4,</t> mCherry-RAB7 proteins, DsRed-RAB9 or DsRed-RAB11. Scale bars: 10 μm. Insets show enlargements of the outlined areas. (B) Quantification. Colocalization between WIPI1[Sloop] and different RAB proteins on the tubular structures was assessed using the Manders’ colocalization coefficient M2, calculated in ImageJ. Data are means ±s.d. of n = 150 cells, from three independent experiments. (C) Colocalization with other endo-lysosomal marker proteins. Cells were fixed 18 h after transfection with EGFP-WIPI1[Sloop], stained with the indicated antibodies and imaged by confocal microscopy. Insets show enlargements of the outlined areas. Scale bars: 10 μm. (D) Quantitative colocalization analysis of the images from (C) was carried out for the tubular structures as in (B). Data are means ± s.d. of n = 160 cells from three independent experiments
Rfp Rab4, supplied by Addgene inc, used in various techniques. Bioz Stars score: 92/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rab4+rfp/pTag-RFP-C-h-Rab4a-c-Myc+(Plasmid+%2379800)/pmc08632285-458-8-7
Average 92 stars, based on 1 article reviews
rfp rab4 - by Bioz Stars, 2026-10
92/100 stars
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rab4 rfp  (OriGene)
90
OriGene rab4 rfp
WIPI1[Sloop] is preferentially associated with recycling endosomes. (A) Colocalization with RAB proteins. Live cell images of HK2 cells expressing EGFP-WIPI1[Sloop], mCherry-RAB5, <t>RFP-RAB4,</t> mCherry-RAB7 proteins, DsRed-RAB9 or DsRed-RAB11. Scale bars: 10 μm. Insets show enlargements of the outlined areas. (B) Quantification. Colocalization between WIPI1[Sloop] and different RAB proteins on the tubular structures was assessed using the Manders’ colocalization coefficient M2, calculated in ImageJ. Data are means ±s.d. of n = 150 cells, from three independent experiments. (C) Colocalization with other endo-lysosomal marker proteins. Cells were fixed 18 h after transfection with EGFP-WIPI1[Sloop], stained with the indicated antibodies and imaged by confocal microscopy. Insets show enlargements of the outlined areas. Scale bars: 10 μm. (D) Quantitative colocalization analysis of the images from (C) was carried out for the tubular structures as in (B). Data are means ± s.d. of n = 160 cells from three independent experiments
Rab4 Rfp, supplied by OriGene, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/product/rab4+rfp/Rab4+(RAB4A)+(NM_004578)+Human+Tagged+ORF+Clone/pmc03993744-52-12-16
Average 90 stars, based on 1 article reviews
rab4 rfp - by Bioz Stars, 2026-10
90/100 stars
  Buy from Supplier
mtagrfp-t-rab4a-7 (plasmid #58025)  (Addgene inc)
N/A
Standard format: Plasmid sent in bacteria as agar stab
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Image Search Results


WIPI1[Sloop] is preferentially associated with recycling endosomes. (A) Colocalization with RAB proteins. Live cell images of HK2 cells expressing EGFP-WIPI1[Sloop], mCherry-RAB5, RFP-RAB4, mCherry-RAB7 proteins, DsRed-RAB9 or DsRed-RAB11. Scale bars: 10 μm. Insets show enlargements of the outlined areas. (B) Quantification. Colocalization between WIPI1[Sloop] and different RAB proteins on the tubular structures was assessed using the Manders’ colocalization coefficient M2, calculated in ImageJ. Data are means ±s.d. of n = 150 cells, from three independent experiments. (C) Colocalization with other endo-lysosomal marker proteins. Cells were fixed 18 h after transfection with EGFP-WIPI1[Sloop], stained with the indicated antibodies and imaged by confocal microscopy. Insets show enlargements of the outlined areas. Scale bars: 10 μm. (D) Quantitative colocalization analysis of the images from (C) was carried out for the tubular structures as in (B). Data are means ± s.d. of n = 160 cells from three independent experiments

Journal: Autophagy

Article Title: WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms

doi: 10.1080/15548627.2021.1886830

Figure Lengend Snippet: WIPI1[Sloop] is preferentially associated with recycling endosomes. (A) Colocalization with RAB proteins. Live cell images of HK2 cells expressing EGFP-WIPI1[Sloop], mCherry-RAB5, RFP-RAB4, mCherry-RAB7 proteins, DsRed-RAB9 or DsRed-RAB11. Scale bars: 10 μm. Insets show enlargements of the outlined areas. (B) Quantification. Colocalization between WIPI1[Sloop] and different RAB proteins on the tubular structures was assessed using the Manders’ colocalization coefficient M2, calculated in ImageJ. Data are means ±s.d. of n = 150 cells, from three independent experiments. (C) Colocalization with other endo-lysosomal marker proteins. Cells were fixed 18 h after transfection with EGFP-WIPI1[Sloop], stained with the indicated antibodies and imaged by confocal microscopy. Insets show enlargements of the outlined areas. Scale bars: 10 μm. (D) Quantitative colocalization analysis of the images from (C) was carried out for the tubular structures as in (B). Data are means ± s.d. of n = 160 cells from three independent experiments

Article Snippet: Vectors expressing tagged-RAB proteins were purchased from Addgene: RFP-RAB4 (79,800; deposited by J.D.

Techniques: Expressing, Marker, Transfection, Staining, Confocal Microscopy

WIPI1-tubules are continuous with endosomes. (A) In HK2 cells expressing EGFP-WIPI1[Sloop], EGFP-labeled tubules (in the boxed area) were photobleached. EGFP fluorescence is shown before and at different times after bleaching. The dashed line indicates the bleached region. The boxed area is shown at higher magnification. Scale bar: 10 μm (B) Quantification of experiments as in a. Means ± s.d. are shown. Data are representative of 3 independent experiments with at least 20 cells analyzed in each experiment. C-H . Experiments as in (A) and (B), respectively, but with cells expressing EGFP-WIPI1[Sloop] plus (C, D) RFP-RAB4, (E, F) DsRed-RAB11 and (G, H) mCherry-RAB5

Journal: Autophagy

Article Title: WIPI1 promotes fission of endosomal transport carriers and formation of autophagosomes through distinct mechanisms

doi: 10.1080/15548627.2021.1886830

Figure Lengend Snippet: WIPI1-tubules are continuous with endosomes. (A) In HK2 cells expressing EGFP-WIPI1[Sloop], EGFP-labeled tubules (in the boxed area) were photobleached. EGFP fluorescence is shown before and at different times after bleaching. The dashed line indicates the bleached region. The boxed area is shown at higher magnification. Scale bar: 10 μm (B) Quantification of experiments as in a. Means ± s.d. are shown. Data are representative of 3 independent experiments with at least 20 cells analyzed in each experiment. C-H . Experiments as in (A) and (B), respectively, but with cells expressing EGFP-WIPI1[Sloop] plus (C, D) RFP-RAB4, (E, F) DsRed-RAB11 and (G, H) mCherry-RAB5

Article Snippet: Vectors expressing tagged-RAB proteins were purchased from Addgene: RFP-RAB4 (79,800; deposited by J.D.

Techniques: Expressing, Labeling, Fluorescence